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BIT 201: Immunochemical Methods
5.00 Credits
Portland Community College
Introduces the general properties adn uses of antibody molecules. Includes an overview immune response, biosynthesis of immunoglobulin, obtain, purifying and labeling antibodies, and using antibodies in a variety of common applications (ELISA, Western blot, immunoprecipitation and immunocytochemistry, antibody-based affinity chromatography). Prerequisite: BIT 109 or 110; BI 112 or CH 100 or higher; or instructor permission.
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BIT 203: Recombinant DNA
5.00 Credits
Portland Community College
Laboratory-intensive course focusing on the strategies and techniques used in recombinant DNA work. Covers vector and insert options and preparation, quantitation of DNA, ligation and transformation procedures, and analysis by restriction digest, blot hybridization and PCR. Prerequisites: BIT 109 or BIT 110; and any of the following: BIT 101, BI 102, BI 112, BI 212 or BI 234, or instructor permission.
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BIT 203 - Recombinant DNA
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BIT 205: Bioseparations
5.00 Credits
Portland Community College
Introduction of commonly used methods for separation of biological molecules for both analytical and preparative applications. This laboratory-intensive course will cover the principles of and practice in filtration, differential precipitation, and electrophoretic and chromatographic techniques. Prerequisite: BIT 109 or BIT 110; and any of the following: BIT 155, BI 112, CH 100 or higher; or instructor permission.
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BIT 207: Cell Culture
5.00 Credits
Portland Community College
Laboratory-intensive course providing introduction to and practice in the culture of animal cells and cell lines. Focus is on routine maintenance and record-keeping, including media preparation, cryopreservation, and troubleshooting common culture problems. Prerequisite: BIT 109 or BIT 110 or instructor permission.
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BIT 211: Biomolecular Principles
4.00 Credits
Portland Community College
Structure/ function relationships of biological molecules. Principles of organic and biochemistry will be related to practical problems of function, detection and separation of biological molecules. Prerequisite: (CH 106 or 223); and BIT 109.
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BIT 211 - Biomolecular Principles
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BIT 215: Protein Purification
5.00 Credits
Portland Community College
Application of commonly used methods for separation of biological molecules in multi-step protein purifications. This laboratory intensive couse will focus on issues of recovery and yield, step-to-step analysis and troubleshooting, as well as documentation and reporting procedures and results. Prerequisite: BIT 205 or instructor permission.
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BIT 217: Tissue Culture II
4.00 Credits
Portland Community College
Second term of a two-term laboratory-intensive course offering more advanced training and practical experience in culture of plant and animal cells. Prerequisite: BIT 207.
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BIT 221: Techniques in Molecular Biology I
5.00 Credits
Portland Community College
First term of a two-term laboratory-intensive course focusing on theory and practice of techniques for analysis and manipulation of nucleic acids. Emphasizes recombinant DNA techniques and strategies and analysis of recombinant DNA by restriction digest, blot hybridization and PCR. Prerequisite: (BI 234 or equivalent), and BIT 109.
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BIT 221 - Techniques in Molecular Biology I
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BIT 223: Advanced DNA Techniques
5.00 Credits
Portland Community College
Laboratory-intensive course focusing on the theory and practice of techniques for analysis and manipulation of nucleic acids. Topics include construction and use of plasmid and phage libraries, DNA sequence determination and analysis, bioinformatics, and applications of PCR. Prerequisites: BIT 203 or instructor permission.
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BIT 280A: Work Experience
8.00 Credits
Portland Community College
Students work in a biotechnology laboratory, supervised by professionals on site and by program instructor(s). Department permission required.
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BIT 280A - Work Experience
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