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Course Criteria
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4.00 Credits
Introduction to molecular biology and protein chemistry. Theory behind laboratory techniques and overview of cloning strategies starting from nucleic acid or protein sequence data. Laboratory sessions involve subcloning, preparation of competent cells, transformation, screening recombinant DNA by colony hybridization and PCR, SDS-PAGE of recombinant protein, affinity purification, and western blots.
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2.00 Credits
Basic techniques in automated DNA sequencing and robotics. Colony picking and ordering cDNA libraries, use of PCR to amplify insert DNA, and strategies for large-scale automated sequencing. Principles of database management for ordering and accessing sequencing information. Half semester course, first part.
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2.00 Credits
Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections
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2.00 Credits
Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part.
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2.00 Credits
Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.
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2.00 Credits
Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, second part.
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2.00 Credits
Introduction to polymerase chain reaction. Optimization of PCR reactions and primer design for DNA sequences using DNA databases available on the web. Laboratory sections include using rapid techniques for isolating and sequencing DNA from small amounts of sample and forensic identification of individuals using isolated human hairs. Credit is not allowed for both BIT 467 and BIT 567.
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2.00 Credits
Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.
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2.00 Credits
Principles of scaling animal cell seed-stock from frozen storage to three liter culture. Students will learn to assemble and operate a three-liter bioreactor to produce antibodies, as well as assess final product quantity using antibody techniques. This is a half-semester course.
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2.00 Credits
Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.
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