BIT 410 - Manipulation of Recombinant DNA

Institution:

North Carolina State University

Subject:

Biotechnology

Description:

Introduction to molecular biology and protein chemistry. Theory behind laboratory techniques and overview of cloning strategies starting from nucleic acid or protein sequence data. Laboratory sessions involve subcloning, preparation of competent cells, transformation, screening recombinant DNA by colony hybridization and PCR, SDS-PAGE of recombinant protein, affinity purification, and western blots.

Credits:

4.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Multiple

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.ncsu.edu

Phone Number:

(919) 515-2011

Regional Accreditation:

Southern Association of Colleges and Schools

Calendar System:

Semester

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