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Course Criteria
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4.00 Credits
Solution and Media Preparation
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4.00 Credits
Introductory course in the theory and concepts of animal cell and tissue culturing. Course will teach the fundamentals in tissue culture techniques, subculturing and maintenance of cell lines. Skills also include: cell viability testing, cell counting, feeding of cell lines and quality control. Aseptic technique is emphasized, along with media preparation. Previously BIOSC 260. Prerequisite: BIOL& 211. Student option grading.
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5.00 Credits
Survey of microorganisms with focus on healthcare applications. Structure, classification, metabolism and genetics of bacteria and viruses are main themes. Emphasis on disease process, microbial control and immunology. Laboratory techniques include isolation and identification of bacteria. Previously BIOSC 215. Prerequisites: BIOL& 211 with minimum 2.0. Student option grading.
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2.00 Credits
Preparation of media and solutions commonly used in biotechnology laboratories. Use of basic lab tools such as pipettors, pH meters, scales, centrifuges, autoclaves and spectrophotometers, and lab notebook maintenance. Previously BIOSC 245. Prerequisite: High school Biology and Chemistry or instructor approval. Student option grading.
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2.00 Credits
Preparation of media and solutions commonly used in biotechnology laboratories. Use of basic lab tools such as pipettors, pH meters, scales, centrifuges, autoclaves and spectrophotometers. Use of MSDS, GLP procedures and lab safety. Previously BIOSC 245. Prerequisite: High school Biology and Chemistry or instructor approval. Student option grading.
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3.00 Credits
This course will focus on DNA replication and translation and regulatory mechanisms in prokaryotic and eukaryotic systems. Previously BIOSC 250. Prerequisite: BIOL& 211 and BIOL 265. Recommended CHEM& 131. Student option grading.
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3.00 Credits
Laboratory emphasis will be on molecular biological techniques utilized in modern research laboratories. Techniques include gene cloning, DNA and protein electrophoresis, protein purification and enzymatic and immunological assays. Previously BIOSC 251. Prerequisite: BIOL& 211 and BIOL 265. CHEM& 131 recommended. Student option grading.
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6.00 Credits
Basic course in the theory and concepts of recombinant DNA techniques. Course will focus on the methods of gene cloning with bacteriophage and plasmid vectors, growth and maintenance of E. coli, extraction and purification of DNA, polymerase chain reaction, southern blots, probe preparation, hybridization methods, and DNA sequencing. The lab will focus on 'hands on' techniques. PreviouslyBIOSC 265. Prerequisite: BIOL 270 or permission. Student option grading.
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6.00 Credits
This course focuses on the concepts and laboratory procedures necessary to understand the cellular, molecular and genetic basis of the immune system. Emphasis will be placed on antigen and antibody structure and function, antibody production, cellular based immunity, and the major histocompatibility complex. Previously BIOSC 270, Prerequisite: BIOL 270 or permission. Student option grading.
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3.00 Credits
A laboratory course which will focus on the techniques currently prevalent in the manufacturing and production aspect of the biotechnology industry. Course will focus on the isolation and purification of proteins. This will include cation-anion exchange chromatography, selective binding using hydrophobic interactions, ultrafiltration, isoelectric focusing and high performance liquid chromatography (HPLC). There will also be exposure to good manufacturing practices (GMP), quality control procedures (QC), biological potency assays, radioisotope use and handling, FDA regulations and clinical trials. Previously BIOSC 275. Prerequisite: BIOL 270 or permission. Student option grading.
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