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Course Criteria
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3.00 Credits
This course provides a basic introduction to the basic life cycle of both drug and medical device life cycles, from discovery through preclinical, clinical trials, post production and post-marketing concerns.
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3.00 Credits
This course will introduce DNA, RNA and proteins and review their structures and functions, including their physical and chemical properties and their roles in cellular metabolism. The course will include an in-depth look at the synthesis of these molecules, as well as DNA replication, transcription and translation.
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3.00 Credits
Presents an overview of microbiology including fundamental structures of microorganisms, their growth, metabolism, interaction with other living things, and classification. Emphasis placed on industrial applications of microbiology.
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2.00 Credits
A conventional laboratory of exercises, demonstrations and discussions. Laboratory exercises are designed to enable students to achieve proficiency in the principles and techniques necessary for cultivation of microorganisms using aseptic techniques and for performing and interpreting biochemical tests. The laboratory exercises will be filled out weekly and turned in to be graded.
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4.00 Credits
Students will study the essential concepts and techniques in genetic engineering. Students will practice essential gene cloning procedures: isolation of DNA, restriction endonuclease digestion, agarose gel electrophoresis analysis, DNA ligation, and transformation into a host strain. Other essential techniques such as PCR, construction and screening of genomic or cDNA libraries, Southern and Northern blot analyses will be practiced. Students will understand the principles and ethical issues of animal or human cloning practices. Current methods for transfer and propagation of genes into plants and animals will be discussed. Various gene knockout techniques such as homologous gene recombination, site-directed mutagenesis, and RNAi will be introduced. The topics in genomics, proteomics, and bioinformatics will be discussed.
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4.00 Credits
Students will be introduced to fermentation processes used for commercial purposes and the operation of small- and large-scale fermentors. Methods used to harvest product from fermentors and the regulatory requirements associated with commercial fermentation will also be explored.
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4.00 Credits
Students will review the biochemical properties of amino acids and proteins, then study techniques of cell disintegration and extraction, protein separation, and analysis. Students will be taught to determine which method is most applicable in various situations and why that method should be utilized. When possible, students will be given an opportunity to perform these techniques in the laboratory.
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3.00 Credits
Presents an in-depth overview of basic biotechnology laboratory skills emphasizing chromatography techniques, methods of DNA and protein electrophoresis, processes of immunoassays, data management skills, recombinant DNA technology, and the polymerase chain reaction
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4.00 Credits
The student will be introduced to basic techniques of plant tissue culture. This is the aseptic culture of plant cells, tissues, organs and plants. This course seeds to familiarize students with the basic principles of tissue culture and to expose them to their many applications. The course includes media preparation, isolation of explants, and establishment of callus from suspension cultures, growth factor bioassays, and regeneration of whole plants from tissue and plant and genetic engineering techniques. We will also discuss the theory, production and societal implications of transgenic plants.
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4.00 Credits
The student will be introduced to biomaterial and tissue functionality and design including the basic concepts underlying physiological responses to wounds and foreign materials. Topics to be considered include biomaterial scaffolds, relevant cell types, soluble regulators or their genes, and mechanical loading and culture conditions. Comparisons will be made between differentiated cell types and stem cells as well as natural and synthetic scaffolds. Methodology for the preparation of cells and scaffolds in practice is described. The rationale for employing growth factors is covered and the techniques for gene modification for optimizing matrix interactions are discussed. Methods for fabricating tissue-engineered products and devices for implantation are taught including material selection and processing, mechanisms of material degradation, cell-material interactions and interfaces, matrix structure transport issues. Examples of tissue engineering ¿based procedures currently employed clinically are analyzed as case studies. Students will gain experience with biomaterial design and modification in addition to cell culture with these matrices.
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