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Course Criteria
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2.00 Credits
Introduction to fermentation and protein chemistry. Theory behind laboratory techniques and overview of industrial scale expression systems. Laboratory session sinvolve use of microbial expression vectors, fermentation systems, and large-scale purification of recombinant protein. Half semester course, first part.
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2.00 Credits
Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part.
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2.00 Credits
Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course.
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2.00 Credits
Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, second part.
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2.00 Credits
Introduction to polymerase chain reaction. Optimization of PCR reactions and primer design for DNA sequences using DAN databases available on the web. Laboratory sections include using rapid techniques for isolating and sequencing DNA from small amounts of sample and forensic identification of individuals using isolated human hairs.
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2.00 Credits
Basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis on agricultural applications. This is a half semester course. Student must register for both the lecture and the lab.
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2.00 Credits
Laboratory-intensive course covering techniques in RNA purification and analysis including: isolation of quality RNA; quantification by gel and spectrophotometer; separation by gel electrophoresis; reverse transcription PCR; and Northern blotting using non-radioactive labeling and detection by chemi-luminescense. Half semester course.
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2.00 Credits
Principles of scaling animal cell seed-stock from frozen storage to three liter culture. Students will learn to assemble and operate a three-liter bioreactor to produce antibodies, as well as assess final product quantity using antibody techniques. This is a half-semester course. Students must register for both lecture and lab.
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2.00 Credits
Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in lants, C. Elegans, and mammalian cell culture. This is a half-semster course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.
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3.00 Credits
Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.
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