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Course Criteria
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4.00 Credits
Lecture, three hours; laboratory, two hours. The use of theories and methods based on computer science, mathematics, and physics in molecular biology and biochemistry. Basics in biomolecular modeling. Analysis of sequence and structural data of biomolecules. Analysis of biomolecular functions. Concurrent with Biological Sciences M123.
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4.00 Credits
Laboratory, four hours. An advanced laboratory for graduate students enrolled in the Biotechnology master's program. Students learn to engineer recombinant eukuryotic viruses and express genes in mouse tissue. Prerequisite: graduate standing.
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2.00 Credits
Seminar and discussion, one hour. Advanced topics in immunology as related to an understanding of human disease. Satisfactory/Unsatisfactory only. May be repeated for credit as topics vary.
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4.00 Credits
An advanced course in genetic engineering and biotechnology for graduate students enrolled in the Biotechnology master's program. Emphasis is placed on learning advanced methods in assembling the gene for expression in bacteria, yeast, and human cells.
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1.00 Credits
Seminar and discussion, one hour. Two half-hour presentations by graduate students and postdoctorals to the department on their current research projects. Satisfactory/Unsatisfactory only. May be taken for credit 15 times.
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4.00 Credits
Lecture, three hours; discussion, one hour. Chemistry of macromolecules; emphasis on proteins. Physical and chemical properties of proteins, forces that maintain protein structure, relationship between structure and function, interactions of proteins with ligands and other macromolecules, and experimental methods to study structure, function, and interactions. Prerequisites: Molecular Biology 203 and 204. Concurrent with Biological Sciences M140. (Coordinators: D. Senear and T. Poulos)
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2.00 Credits
Lecture, two hours. Taken concurrently with 250L, supplements laboratory curriculum with scientific background behind experimental methods. Format consists of lectures and the presentation and analysis of relevant papers from the scientific literature. Corequisite: Molecular Biology and Biochemistry 250L. Limited to Biotechnology concentration M.S. students or consent of instructor.
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8.00 Credits
Laboratory, eight hours. Nucleic acid techniques and recombinant DNA technology. Extraction and purification of nucleic acids, cloning and subcloning, PCR, site-directed mutagenesis, nucleic acid hybridization, additional associated procedures. Students must demonstrate accurate documentation of data (laboratory notebook) detailing experience and results. Corequisite: Molecular Biology and Biochemistry 250. Limited to Biotechnology concentration M.S. students or consent of instructor.
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2.00 Credits
Lecture, two hours. Taken concurrently with 251L, supplements laboratory curriculum with scientific background behind experimental methods. Format consists of lectures and the presentation and analysis of relevant papers from the scientific literature. Corequisite: Molecular Biology and Biochemistry 251L. Prerequisites: Molecular Biology and Biotechnology 250 and 250L. Limited to Biotechnology concentration M.S. students or consent of instructor.
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8.00 Credits
Laboratory, eight hours. Major techniques of handling proteins and antibodies. Protein engineering, expression and large-scale purification of recombinant proteins from bacteria, HPLC, antibody purification, western blotting, additional associated procedures. Students must demonstrate accurate documentation of data (laboratory notebook) detailing experience and results. Corequisite: Molecular Biology and Biochemistry 251. Prerequisites: Molecular Biology and Biotechnology 250 and 250L. Limited to Biotechnology concentration M.S. students or consent of instructor.
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