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Course Criteria
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6.00 Credits
This course teaches the skills needed to serve as a technician in biotechnology production. Students grow and monitor bacterial, yeast, and mammalian cells on a laboratory scale that emulates the large-scale production used in industry. Students will become familiar with the cleaning, sterilization, aseptic inoculation, operation, and monitoring of fermenters and bioreactors. Students then recover and purify proteins produced by those cell cultures. They recover and purify proteins using centrifugation, ultrafiltration, and chromatography techniques. The course emphasizes the use of current Good Manufacturing Practices (cGMP), and students gain experience following Standard Operating Procedures (SOP). Prerequisites: BTEC 51A. Two hours lecture, nine hours laboratory GE Area: Non-GE Applicable
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6.00 Credits
This course familiarizes students with small scale laboratory practices, both those used in a research laboratory and those used by a quality control department in industry, to analyze the quality of a cell culture process and the purity of protein products produced by cells in culture. The course emphasizes the use of Good Laboratory Practices (GLP) in these analyses. Students will gain experience in techniques used to analyze nucleic acids and in the genetic engineering of cells. They will also gain experience with the common assays used in Quality Control including electrophoresis, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbant Assay (ELISA), and Polymerase Chain Reaction (PCR) to test products generated using cell culture. Prerequisites: BTEC 51A. Two hours lecture, nine hours laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Understanding, using, and performing electrophoretic separations and transfers in a research or industrial setting. This is to include the molecular and physical basis of specific techniques, and their practical applications. Techniques covered will include gel electrophoresis, capillary electrophoresis, isoelectric focusing, 2D gels and electrotransfers. The applications of these techniques for proteins, carbohydrates and small molecules, within research and industry will bepresented. The instrumentation used for electrophoresis, isoelectric focusing, and capillary electrophoresis and practical experience with reagents and instrumentation will be emphasized. Students will follow established protocols, and demonstrate an understanding of supporting routine operations and standard protocols. Prerequisites: Laboratory experience (high school and/or professional experience). Advisory: BTEC 64 and BIOL 64 are interchangeable. High school biology, chemistry, and algebra recommended. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Understanding, using, and performing electrophoretic separations and transfers in a research or industrial setting. This is to include the molecular and physical basis of specific techniques, and their practical applications. Techniques covered will include gel electrophoresis, capillary electrophoresis and electrotransfers. The applications of these techniques for proteins, and small molecules, within research and industry will be presented. The instrumentation used for electrophoresis, capillary electrophoresis, and pulsed gel electrophoresis and practical experience with reagents and instrumentation will be emphasized. Students will follow established protocols, and demonstrate an understanding of supporting routine operations and standard protocols. Prerequisites: Laboratory experience (high school and/or professional experience). Advisory: BTEC 65 and BIOL 65 are interchangeable. High school biology, chemistry, and algebra recommended. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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2.00 Credits
Understanding, using and performing HPLC in a research or industrial setting. Includes the theory and mechanisms of molecules and chemistry, the wide range of research, analytical and preparative uses, instrumentation used for HPLC, practical experience with reagents and instrumentation, following established protocols, calibrating and maintaining the instrumentation. Prerequisites: High school biology, chemistry and algebra; laboratory experience. Advisory: BTEC 66 and BIOL 66 are interchangeable. Repeatability: May be taken two times for credit. Four hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Understanding and performing immunological assays. Includes the theory, molecular basis, and research/diagnostic applications of several techniques. Techniques covered will include, direct, indirect, sandwich, and quantitative ELISAs, and Western blotting. Practical experience with reagents (selection of conjugated antibodies, detection systems) and instrumentation (microtiter plate reader, polyacrylamide gel electrophoresis apparatus, transfer apparatus) will be emphasized. Prerequisites: Laboratory experience (high school, college and/or professional); high school chemistry, biology, algebra. Advisory: BTEC 67 and BIOL 67 are interchangeable. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Understanding, using and performing PCR in a research or industrial setting. Includes the molecular and physical basis of the technique, mechanisms and practical (research and analytical) applications, RT-PCR, product separation and detection, thermocyclers, primers, practical experience with reagents and instrumentation for PCR, following established protocols. Prerequisites: Laboratory experience (high school, college and/or professional); high school chemistry, biology, algebra. Advisory: BTEC 68 and BIOL 78 are interchangeable. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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3.00 Credits
Introduction to general mammalian cell culture techniques, including media preparation, sterile technique, freezing, thawing, and maintaining primary cell and cell lines. Theoretical considerations will include purpose and selection of media components, setting up and maintaining a sterile cell culture environment, and controlling contamination. Students will gain practical experience working in the laminar flow hood, counting cells, isolating cells from a primary source, and maintaining healthy adherent and suspension cells in culture. Emphasis will also be given to proper care and use of equipment used in a cell culture facility: laminar flow hoods, CO2 incubators, water baths, and the inverted microscope. Prerequisites: Laboratory experience (high school, college and/or professional). Advisory: High school chemistry, biology, algebra recommended. Repeatability: May be taken two times for credit. Six hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Production of monoclonal antibodies by hybridoma technology. Course will include theoretical discussion of therapeutic and diagnostic uses of antibodies, sterile technique, hybridoma production, selection, and cell cloning. Students will gain practical experience of hybridoma technology by performing a cell fusion, screening and selecting positive hybridomas, and cloning cells to isolate monoclonal antibodies. A brief discussion of the ELISA (enzyme-linked immunosorbent assay) will be included. Prerequisites: Laboratory experience (high school, college and/or professional). Advisory: BTEC 70 and BIOL 80 are interchangeable. BTEC 53A, BTEC 69 and animal cell culture experience. High school chemistry, biology, algebra. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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2.00 Credits
Understanding, using and performing DNA sequencing and cloning techniques in a research and production setting. Includes applications of cDNA and PCR product sequencing, historical and theoretical basis of conventional and automated DNA sequencing, experimental design of sequencing methods, oligonucleotide synthesis, construction of sequencing and expressions plasmids, and vectorology. Laboratory exercises will involve DNA and RNA manipulation using established protocols and computer assisted methods (bioinformatics). Prerequisites: Laboratory experience (high school and/or professional experience). Advisory: BTEC 71 and BIOL 71 are interchangeable. High school biology, chemistry, and algebra recommended. Repeatability: May be taken two times for credit. Four hours lecture-laboratory. GE Area: Non-GE Applicable
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