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Course Criteria
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4.00 Credits
Introduction to the medical aspects of nutrition. Biological function and chemical classification of nutrients. Nutritional needs throughout the lifespan. Effects of nutritional deficiencies and excesses. Recommended nutrient intakes and the role of diet in the development of chronic disease. Prerequisites: CHEM 30A, or 1 year of high school chemistry, and BIOL 40A, 40B, and 40C (BIOL 40C may be taken concurrently). Advisory: ENGL 1A or ESL 26. Four hours lecture. GE Area: Lifelong Understanding
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4.00 Credits
General principles of pharmacology with emphasis on drug-receptor interactions, second messenger systems, determinants of drug response, pharmacokinetics, bio transformation and excretion, pharmacogenetics, drug development and legal aspects of drug distribution. Pharmacology of the autonomic nervous system. Application of pharmacological principles and concepts with emphasis on the various pharmacological classes of drugs in diverse patient populations. Prerequisites: CHEM 30B, and BIOL 40A, 40B, 40C (BIOL 40C may be taken concurrently). Advisory: ENGL 1A or ESL 26 recommended. Four hours lecture. GE Area: Non-GE Applicable
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1.00 Credits
Understanding, using, and performing electrophoretic separations and transfers in a research or industrial setting. This is to include the molecular and physical basis of specific techniques, and their practical applications. Techniques covered will include gel electrophoresis, capillary electrophoresis, isoelectric focusing, 2D gels and electrotransfers. The applications of these techniques for proteins, carbohydrates and small molecules, within research and industry will be presented. The instrumentation used for electrophoresis, isoelectric focusing, and capillary electrophoresis and practical experience with reagents and instrumentation will be emphasized. Students will follow established protocols, and demonstrate an understanding of supporting routine operations and standard protocols. Prerequisite: Laboratory experience (high school and/or professional experience). Advisories: High school biology, chemistry, algebra recommended. BIOL 64 and BTEC 64 are interchangeable. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Understanding, using, and performing electrophoretic separations and transfers in a research or industrial setting. This is to include the molecular and physical basis of specific techniques, and their practical applications. Techniques covered will include gel electrophoresis, capillary electrophoresis and electrotransfers. The applications of these techniques for proteins, and small molecules, within research and industry will be presented. The instrumentation used for electrophoresis, capillary electrophoresis, and pulsed gel electrophoresis and practical experience with reagents and instrumentation will be emphasized. Students will follow established protocols, and demonstrate an understanding of supporting routine operations and standard protocols. Prerequisite: Laboratory experience (high school and/or professional experience). Advisories: High school biology, chemistry, algebra recommended. BIOL 65 and BTEC 65 are interchangeable. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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2.00 Credits
Understanding, using and performing HPLC in a research or industrial setting. Includes the theory and mechanisms of molecules and chemistry, the wide range of research, analytical and preparative uses, instrumentation used for HPLC, practical experience with reagents and instrumentation, following established protocols, calibrating and maintaining the instrumentation. Prerequisites: High school biology, chemistry and algebra; laboratory experience. Advisory: BIOL 66 and BTEC 66 are interchangeable. Repeatability: May be taken two times for credit. Four hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Understanding and performing immunological assays. Includes the theory, molecular basis, and research/diagnostic applications of several techniques. Techniques covered will include, direct, indirect, sandwich, and quantitative ELISAs, and Western blotting. Practical experience with reagents (selection of conjugated antibodies, detection systems) and instrumentation (microtiter plate reader, polyacrylamide gel electrophoresis apparatus, transfer apparatus) will be emphasized. Prerequisites: Laboratory experience (high school, college and/or professional); high school chemistry, biology, algebra. Advisory: BIOL 67 and BTEC 67 are interchangeable. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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3.00 Credits
Introduction to general mammalian cell culture techniques, including media preparation, sterile technique, freezing, thawing, and maintaining primary cells and cell lines. Theoretical considerations will include purpose and selection of media components, setting up and maintaining a sterile cell culture environment, and controlling contamination. Students will gain practical experience working in the laminar flow hood, counting cells, isolating cells from a primary source, and maintaining healthy adherent and suspension cells in culture. Emphasis will also be given to proper care and use of equipment used in a cell culture facility: laminar flow hoods, CO2 incubators, water baths, and the inverted microscope. Prerequisites: Laboratory experience (high school, college and/or professional). Advisory: BIOL 69 and BTEC 69 are interchangeable. High school chemistry, biology, algebra. Repeatability: May be taken two times for credit. Six hours lecture-laboratory. GE Area: Non-GE Applicable
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2.00 Credits
Understanding, using and performing DNA sequencing and cloning techniques in a research and production setting. Includes applications of cDNA and PCR product sequencing, historical and theoretical basis of conventional and automated DNA sequencing, experimental design of sequencing methods, oligonucleotide synthesis, construction of sequencing and expressions plasmids, and vectorology. Laboratory exercises will involve DNA and RNA manipulation using established protocols and computer assisted methods (bioinformatics). Prerequisites: Laboratory experience (high school and/or professional experience). Advisory: BIOL 71 and BTEC 71 are interchangeable. High school biology, chemistry, algebra recommended. Repeatability: May be taken two times for credit. Four hours lecture-laboratory. GE Area: Non-GE Applicable
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2.00 Credits
Understanding, using and performing HPLC in a research or industrial setting. Includes the theory and mechanisms of molecules and chemistry, the wide range of research, analytical and preparative uses, instrumentation used for HPLC, practical experience with reagents and instrumentation, following established protocols, calibrating and maintaining the instrumentation. Prerequisites: High school biology, chemistry and algebra; laboratory experience, successful completion of BTEC 66 or equivalent experience. Advisory: BIOL 72 and BTEC 72 are interchangeable. Four hours lecture-laboratory. GE Area: Non-GE Applicable
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1.00 Credits
Introduction to basic histotechnology techniques, including solution preparation, fixation, processing, embedding, thin and thick sectioning, and staining. Learn how histology aids in disease detection and explore how it is used as a tool in research. Prerequisites: Laboratory experience (high school and/or professional experience). Advisory: BIOL 73 and BTEC 73 interchangeable. High school biology, chemistry, algebra recommended. Repeatability: May be taken two times for credit. Two hours lecture-laboratory. GE Area: Non-GE Applicable
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