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Course Criteria
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2.00 Credits
Lec-36, lab-18 (total hrs), field trips P/NP available PREREQ.: BTEC 21 or 21A Introduction to advanced techniques in the study of normal and mutant tissue culture cells including organelle visualization with various fluorophores, transfection with fluorescent markers, and immunostaining. In depth coverage of the theory behind and use of fluorescent microscopy and current research methods using fluorescent technology, including apoptosis assays and immunohistochemistry, DNA microarrays and FACS analysis. CSU
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3.00 Credits
Lec-48, lab-24 (total hrs), field trips P/NP available PREREQ.: BTEC 21B A general introduction to the principles of stem cell biology. Topics include embryonic stem cells in early development, adult stem cells, potential applications of stem cell culture and ethical issues involved in stem cell research. Emphasis on laboratory techniques including proper use and care of equipment, flow cytometry, isolation of mouse adult stem cells, and culture of mouse embryonic stem cells. CSU
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1.00 Credits
Lec-14, lab-10 (total hrs), field trips P/NP available Underlying principles of immunoassay with focus on ELISA. Examples of new procedures, technical advances, past, present and future prospects in the pharmaceutical, biotechnology and clinical laboratory setttings will be discussed. Implications for society and governmental regulations will be addressed. Course will feature hands on exercises emphasizing all aspects of ELISA. CSU
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2.00 Credits
Lec-1, lab-3 PREREQ.: ET 108A or MATH 840 or demonstration of ET 108A or MATH 840 exit skills An introduction to the theory and application of process control and instrumentation as applied to the Biotechnology Industry. Survey of Electrical and Electronic concepts. Transducers. Process Controllers. Reliability. Data Loggers. CSU BTEC 221=ELEC 221
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1.00 Credits
Lec-9, lab-27 (total hours) PREREQ.: BTEC 221 An introduction to the theory and application of calibration and validation as applied to the Biotechnology Industry.CSU
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1.00 Credits
Lec-16, lab-8 (total hrs), field trips P/NP available Underlying principles of immunoassays with focus on Western blotting. Examples of new procedures, technical advances, past, present and future prospects in the pharmaceutical, biotechnology and clinical laboratory settings will be discussed. Implications for society and governmental regulations will be addressed. Course will feature hands-on laboratory exercises emphasizing all aspects of Western blotting. CSU
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0.50 Credits
Lec-12, lab-8 (total hrs), field trips P/NP available An introduction to the theoretical aspects and laboratory techniques of the Polymerase Chain Reaction (PCR). Students gain practical experience performing PCR as well as experimenting with optimization of the reaction. Applications of PCR used in DNA fingerprinting, diagnostic medicine, genome mapping, evolutionary relationship determination, and epidemiology will be discussed. CSU
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1.00 Credits
Lec-16, lab-8 (total hrs), field trips P/NP available PREREQ.: Completion of BTEC 24 or demonstration of BTEC 24 exit skills A course in the theory and practice of current polymerase chain reaction (PCR) analytical methods. Students will gain laboratory experience in performing techniques such as reverse transcriptase PCR (RT-PCR), real-time PCR, inverse PCR, construction of oligonucleotide DNA microarrays, site-directed mutagenesis, and strategies for cloning genes from organisms lacking genome projects. Experimental optimization will be emphasized. CSU
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0.50 Credits
Lec-16, lab-8 (total hrs), field trips P/NP available Advise: BIO 11 or 101A or 65 An advanced course in molecular biology techniques that extends on topics covered in Biology 65. Students will become proficient in the skills of Southern and Northern blotting. Students will isolate nucleic acid (DNA and RNA), prepare them for the appropriate agarose gel electrophoresis protocols, prepare DNA probes, set-up nucleic acid hybridizations, and complete and interpret non-radioactive Southern and Northern blots. CSU
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1.00 Credits
Lec-2 (9 wks), field trips .. .. .. .. .. .. .. .. ..P/NP available Underlying principles of molecular biology and the laboratory procedures involved in the biotechnology industry. Examples of new products, processes, and prospects for the future in medicine, environmental restoration, forensics, and agriculture. Implications for society and governmental regulations. Features lectures by scientists currently working in the biotechnology industry. CSU Formerly BIO 50.
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