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  • BI 220: Immunology

    4.00 Credits
    Massachusetts Bay Community College

    Prerequisite: BI 210. This course emphasizes the molecular and cellular interactions involved in immune responses. Topics covered include antibody structure and function; applications of monoclonal antibodies in medicine; gene rearrangements in B and T cells; cellular cooperation and role of the MHC; tolerance; and immunopathology (hypersensitivity, autoimmunity, transplantation, cancer immunity and immunotherapy, and AIDS). Laboratory involves antibody purification, immunoprecipitation assays, immunoblotting, and ELISAs (indirect, sandwich, and competitive). Lecture: 3 hours per week. Lab: 3 hours per week. 4 credits
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  • BT 101: Rotation I: Introduction to Biotechnology

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites/Co-requisites: CH 110, BI 110. In this hands-on laboratory course students are introduced to the scientific method and key biotechnology methodologies through their participation in an ongoing molecular research project(s) conducted by the Biotechnology Program called the Grand Project. The research and training emphasis will be on protein and nucleic acid analysis of cellular proteins using the electrophoresisbased methodologies: Western-, Northern-, and Southern-blot hybridization analysis. Students will also be introduced to mammalian cell culture. Lab: 6 hours per week. 2 credits
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  • BT 107: Forensic Dna Science I

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 110, BI 110, BT 101. In this hands-on laboratory course students learn DNA typing and allele frequency analysis through the most commonly used methodologies for human identification in criminal cases involving DNA evidence including: Co-Filer? and Pro-Filer Plus?, Ident-Filer? and other allelic analysis methods. Students will develop and refine their DNA analysis skills by their direct involvement in actual criminal- and/ or cold-cases. Students will be graded based on their performance in mock testimony in a moot court under cross-examination by a lawyer or law student in which the student must defend his/her forensic DNA findings. Lab: 6 hours per week. 2 credits
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  • BT 108: Marine Rotation I

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 110, BI 110, BT 101. This course familiarizes the student with the fundamentals of marine boating, in particular as it relates to field research and qualifies them for certification in most states that require boating education before operating a watercraft. Subjects include: boat handling under normal conditions, adverse conditions and emergencies; research diving, underwater transect deployment and use: types of boats and boating terminology; required and recommended boat equipment; boating regulations and navigation rules; lines and knots; charts and aids to navigation; piloting techniques; and marine radiotelephone usage. Lab: 6 hours per week. 2 credits
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  • BT 201: Rotation II: Mammalian Cell Culture

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 110, BI 110, BT 101. In this hands-on laboratory course students are trained to establish and maintain mammalian cell lines and perform sophisticated molecular experiments in mammalian cells systems. Proficiency in cell culture will be conferred through the student's semester-long advancement of a molecular sub-project to be assigned by the instructor. Each sub-project will converge on the ongoing Grand Project conducted by the Biotechnology Program. Through their individual research sub-projects students will learn to apply the key molecular methodologies learned in Rotation I to addressing real scientific problems through the use of cell systems. Research training emphasis will be placed on extraction, purification and analysis of cellular proteins and nucleic acids, transfection, short-term and long-term cell storage, and cell fusion. Lab: 6 hours per week. 2 credits
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  • BT 205: Forensic Science III

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 120, BI 110, BT 107, BT 201. This is a highly rigorous, hands-on laboratory course in which students learn mitochondrial DNA (mtDNA) analysis of human remains and human mtDNA haplotyping. Emphasis will be placed on the sequencing and characterization of the hypervariable region (HVr)-1 and HVr-2 of human mtDNA for purposes of establishing identity, determining ethnic origin, and linking remains to maternal family lineages. Students will be trained by their involvement in actual criminal cold-cases, missing person cases or anthropological cases. Students will be graded based on their performance in mock testimony in a moot court under cross-examination by a lawyer or law student in which the student must defend his/her forensic DNA findings. Lab: 6 hours per week. 2 credits
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  • BT 206: Marine Rotation II

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 120, BI 120, BT 108. This courses teaches the student basic marine navigation and emphasizes the basics of coastal and inland navigation. The course also embraces GPS as a primary navigation and research tool while covering enough of traditional techniques (such as "bearings" and "dead-reckoning?o the student will be able to find his/her way even if their GPS fails. The course includes many in-class exercises and one open-water skills exercise, developing the student's navigational proficiency through hands-on practice and learning. Lab: 6 hours per week. 2 credits
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  • BT 211: Rotation III: Protein Analysis

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 110, BI 110, BT 101. In this hands-on laboratory course students learn the isolation, purification and characterization of cellular proteins from kilogram to nanogram scales. Emphasis will be placed on training the student in protein characterization: peptide mapping, amino acid analysis, ultrafiltration, low and medium pressure and high performance liquid chromatography (HPLC), gel filtration, ion exchange, reverse phase and affinity chromatography. Lab: 6 hours per week. 2 credits
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  • BT 215: Forensic Dna Science III

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 120, BI 110, BT 107, BT 201. This is a highly rigorous, hands-on laboratory course in which students learn mitochondrial DNA (mtDNA) analysis of human remains and human mtDNA haplotyping. Emphasis will be placed on the sequencing and characterization of the hypervariable region HVr1, HVr2 and HVr3 of human mtDNA for purposes of establishing identity, determining ethnic origin, and linking remains to maternal family lineages. Students will be trained by their involvement in actual criminal cold-cases, missing person cases or anthropological cases. Students will be graded based on their performance in mock testimony in a moot court under cross-examination by a lawyer or law student in which the student must defend his/her forensic DNA findings. Lab: 6 hours per week. 2 credits
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  • BT 221: Biotechnology Rotation IV

    2.00 Credits
    Massachusetts Bay Community College

    Prerequisites: CH 210, BI 120, BT 211. In this course students demonstrate their ability to conduct independent molecular research through their formulation and advancement of a semester-long project that addresses an original scientific question. Students first submit to the instructor a 5-page research proposal outlining research goals and methodologies to be employed before the third week of class. Students will be graded on the scientific quality of their research data as demonstrated in a 20-minute power-point presentation before a panel of scientists selected by the instructor. Grades will be assigned by the instructor and based on the feedback of the panel and the quality of the student's data. Lab: 6 hours per week. 2 credits
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