ENVE 6001 - Applied Biology for Engineers Laboratory

Institution:

University of Cincinnati-Main Campus

Subject:

Description:

The lab portion of Applied Biology for Engineers will mirror the topics covered in lecture starting with DNA structure, primer design and PCR. Students will design and perform a PCR reaction, and clone a gene of interest into a recombinant bacterial expression strain. A small scale growth procedure, including media preparation and affinity tag purification of the recombinant protein will be included. Finally, the purified enzyme will be tested for performance and kinetics. Operational knowledge of equipment and basic lab skills will be obtained, and a detailed lab notebook representing the culmination of the lab course work will be evaluated for the grade.

Credits:

2.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.uc.edu

Phone Number:

(513) 556-6000

Regional Accreditation:

North Central Association of Colleges and Schools

Calendar System:

Semester

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