BIOL-UA 130 - At the Bench: Biological Chemistry–Genomes to Molecular Machines

Institution:

New York University

Subject:

Description:

An upper-level elective lab course for students majoring in biology and those seeking to fulfill requirements for entrance into advanced degree programs. Using biochemical and genetic approaches with the yeast Saccharomyces cerevisiae, students characterize a large multisubunit protein complex that modifies chromatin and is involved in gene regulation. S. cerevisiae is a unicellular eukaryote, better known as baker's yeast, that is a widely used biochemical and genetic model organism. Affinity chromatography is used to produce purified preparations of wild-type and mutant protein complexes. The purified protein complexes are compared using a wide variety of biochemical techniques, including silver-stained SDS-PAGE, western blot, colorimetric enzymatic assay, and protein interaction assays. Yeast expressing the same mutants is used in genetic experiments to evaluate the importance of the protein complex in cell growth and gene regulation in the cell.

Credits:

4.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.nyu.edu

Phone Number:

(212) 998-1212

Regional Accreditation:

Middle States Association of Colleges and Schools

Calendar System:

Semester

The Course Profile information is provided and updated by third parties including the respective institutions. While the institutions are able to update their information at any time, the information is not independently validated, and no party associated with this website can accept responsibility for its accuracy.