BIOS 1032 - Quantitative Imaging of Cells

Institution:

Yeshiva University

Subject:

Biomedical Sciences (Graduate)

Description:

1032 QUANTITATIVE IMAGING OF CELLS COURSE LEADERS: Drs. Ben Ovryn and Erik Snapp CREDITS/CLASS MEETINGS: 3 semester hours; Lectures: 1.5 hrs, twice a week plus laboratories: 1.5 hrs, once per week. RECOMMENDED BACKGROUND: Undergraduate courses in Biochemistry, Cell Biology, Physics, Algebra, Statistics, and Trigonometry. COURSE TEXT: Fundamentals of Light Microscopy and Electronic Imaging by Douglas B. Murphy Methods in Cell Biology. Volume 81. Digital Microscopy: 3rd Edition Ed. by Greenfield Sluder and David E. Wolf. Course notes and additional materials to be assigned. SUITABILITY FOR 1ST YEAR STUDENTS: All graduate and MSTP students may enroll. ENROLLMENT: Enrollment for the course is limited to approximately 15 students due to restricted lab space. Preference will be given to 2nd year and above graduate and MSTP students with a quantitative imaging component to their projects. NOTE: This is a closed registration and you must get approval from the course leaders in order to register for the course. COURSE DESCRIPTION: This course presents an in-depth analysis of the principles and applications of light microscopy as applied to imaging cellular structures and molecules. Topics will include: the essentials of light microscopy; fluorophores; live cell imaging; deconvolution; confocal microscopy; photomanipulation; fluorescence correlation spectroscopy, multi-photon intravital imaging; evanescent wave imaging; fluorescence energy transfer and fluorescence lifetime imaging; FACS and evolving microscopies. Formal lectures will be supplemented with laboratory demonstrations that use state-of-the-art microscopes. Grading will be based upon a combination of laboratory exercises, problem sets, midterm, and a final exam.

Credits:

3.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.yu.edu

Phone Number:

(212) 960-5400

Regional Accreditation:

Middle States Association of Colleges and Schools

Calendar System:

Semester

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