BCH 208 - Tech In Bch & Molecular Bio

Institution:

University of Rochester

Subject:

Description:

A laboratory course designed to introduce students to the theory and practice of biochemical, computational and molecular biology techniques. Students will gain first-hand experience with these techniques, instrumentation and computational methods for macromolecular analysis. Topics which will be covered are: agarose gel and SDS polyacrylaminade gel electrophoresis; computer assisted protein structure analysis, RNA secondary structure analysis, DNA sequence database searching and DNA sequence alignment; DNA isolation; DNA cloning; DNA restriction fragment analysis; polymerase chain reaction; laboratory safety; transformation of bacteria; expression of recombinant proteins; transfection of mammalian cells; subcellular trafficking of proteins; fractionation and purification of proteins; affinity chromatography, enzyme reactions and measuring kinetic parameters; spectrophotometry; RNA secondary structure, DNA and protein quantification and western blotting.

Credits:

4.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.rochester.edu

Phone Number:

(888) 822-2256

Regional Accreditation:

Middle States Association of Colleges and Schools

Calendar System:

Semester

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