BIOL 507 - Recombinant DNA Techniques

Institution:

Duquesne University

Subject:

BIOL - Biology

Description:

This course provides a broad hands-on introduction to contemporary techniques in molecular biology. Manipulation of cloned DNA using restriction endonucleases, ligases, and phosphatases will be carried out. Molecular cloning using both plasmid and bateriophage vectors will be done. Nucleotide sequencing and sequence analysis will be discussed. The student will carry out a nucleic acid hybridization in the form of a Southern blot. Cloned DNA will be transposon mutagenized. Analysis of cloned DNA using a reporter gene will be carried out. DNA amplification using the Polymerase Chain reaction will be done.

Credits:

4.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture/Lab

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.duq.edu

Phone Number:

(412) 396-6000

Regional Accreditation:

Middle States Association of Colleges and Schools

Calendar System:

Semester

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