1001-315 - Hybridoma Techniques

Institution:

Rochester Institute of Technology

Subject:

Description:

This course is designed to acquaint each student with the basic methods employed in the production of hybridoma cell lines and monoclonal antibodies. Includes preparation of viable cell suspensions, lymphocyte-myeloma cell fusion using polyethylene glycol, selection for and culturing of hybridoma cells, cloning by limiting dilution, ELISA, immunization of mice, monoclonal antibody production and scale up of hybridoma cells. (1001-311 and 1001-314 required; 1001-312 recommended) Lab 4, Credit 2 (S)

Credits:

4.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.rit.edu/

Phone Number:

(585) 475-2411

Regional Accreditation:

Middle States Association of Colleges and Schools

Calendar System:

Quarter

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