MICR 230 - Molecular Biology KCC AA/DB

Institution:

Kapiolani Community College

Subject:

Description:

6 hours lecture/lab per week Prerequisite(s): MICR 130, MICR 135 or BIOL 171; MICR 140, MLT 107 or BIOL 171L; CHEM 151 or higher; CHEM 151L or higher. MICR 230 covers the fundamental theoretical and laboratory aspects of molecular biology. The basic principles which govern the structure and function of proteins, nucleic acids and macromolecular complexes will be studied. Students will learn and become proficient at performing the fundamental laboratory procedures of biotechnology. Upon successful completion of MICR 230, the student should be able to: Describe the structure of proteins, nucleic acids and macromolecular complexes. Describe the function of nucleic acids, proteins and macromolecules in DNA replication, transcription, translation, mutagenesis and DNA repair. Describe the regulation of gene activity in prokaryotes and eukaryotes. Describe basic principles and techniques of molecular biology including the use of plasmids and transposons to generate recombinant DNA. Prepare, sterilize and dispense the basic types of media used for the cultivation of bacteria. Operate all the basic equipment of a molecular biology laboratory, including but not limited to large autoclaves and bench top autoclaves, water distillation apparatus, biological safety cabinets, spectrophotometers and ELISA readers, electrophoresis equipment, centrifuges and microcentrifuges. Perform agarose gel electrophoresis. Isolate and quantitate chromosomal and plasmid DNA from bacteria. Perform and analyze restriction enzyme digestions of DNA. Prepare and screen a genomic library. Prepare enzyme labeled probes and perform southern blots. Perform polymerase chain reactions under a variety of conditions. Analyze DNA and amino acid sequence data by searching sequence data bases.

Credits:

3.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

kapiolani.hawaii.edu/page/home

Phone Number:

(808) 734-9000

Regional Accreditation:

Western Association of Schools and Colleges

Calendar System:

Semester

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