MICR 240 - Cell Biology and Tissue Culture KCC AA/DY

Institution:

Kapiolani Community College

Subject:

Description:

4 hours lecture/lab per week Prerequisite(s): MICR 130 or MICR 135 or BIOL 171; MICR 140 or MLT 107 or BIOL 171L; CHEM 151/151L or higher level chemistry course with lab; or instructor's consent. Comment: MICR 240 is cross-listed as BIOL 275L. MICR 240 is a lecture/laboratory course that covers cell biology and the essential principles important to the cultivation and study of cells in tissue culture. Through lectures and laboratory experiments students will acquire a fundamental understanding of the biochemistry and molecular biology of the cell. Students will also acquire competence in tissue culture and experience with modern advances in biotechnology and recombinant DNA technology. Upon successful completion of MICR 240 the student should be able to: Prepare media and buffers needed for the culture of animal, plant and microalgae cells. Demonstrate proficiency in the specialized sterilization and quality control procedures used in a tissue culture laboratory. Demonstrate proficiency in routine cell culture protocols such as feeding schedules and medium supplements, subcultivation procedures, cell enumeration and viability testing, cryopreservation, and the detection and disposition of contaminated cultures. Demonstrate knowledge of the basic principles of protein chemistry by applying these principles in the designing and reporting of experiments utilizing enzymatic reactions, electrophoresis and immunoassays. Demonstrate knowledge of the basic principles of DNA structure, function, and chemistry by applying these principles in the designing and reporting of experiments utilizing DNA extraction and purification, electrophoresis, restriction enzyme analysis, DNA amplification, sequencing, and sequence analysis using standard bioinformatics databases and analysis protocols. Describe in detail the organization of life at the cellular and subcellular levels. 9 Describe the structure and function of biological membranes and demonstrate an understanding of the processes which occur at the cell surface. Describe in detailed and specific terms the fundamental catabolic and anabolic metabolic processes that occur at the cellular level. Describe and experimentally manipulate the cytoskeleton particularly as it relates to intracellular traffic, cytokinesis and cell motility. Describe and experimentally manipulate the basic processes involved in cell signaling and the cell cycle and define the role of these processes in cell differentiation and in cancer. Describe the theories explaining the development of eukaryotes and the evolution of multicellular organisms. Describe and debate the ethical issues surrounding existing and proposed research and applications using living cells.

Credits:

2.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

kapiolani.hawaii.edu/page/home

Phone Number:

(808) 734-9000

Regional Accreditation:

Western Association of Schools and Colleges

Calendar System:

Semester

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