MICR 161 - Immunology and Protein Chemistry

Institution:

Kapiolani Community College

Subject:

Description:

4 hours lecture/lab per week Prerequisite(s): Credit or concurrent enrollment in MICR 130, MICR 135 or BIOL 171; credit or concurrent enrollment in MICR 140, MLT 107 or BIOL 171L; credit or concurrent enrollment in CHEM 151 or higher level chemistry course; credit or concurrent enrollment in CHEM 151L or higher level chemistry course. MICR 161 lecture/laboratory course covers the fundamental aspects of both immunology and protein chemistry as it is performed in clinical and biotechnology laboratories. Upon successful completion of MICR 161, the student should be able to: Describe the structure and chemistry of proteins, with especial emphasis on the immunoglobulins. Describe and explain the principles underlying antigenantibody reactions. Demonstrate proficiency in performing a variety of immunoassays including agglutination, precipitation, ELISA, and fluorescent antibody procedures. Explain the principles of electrophoresis and perform polyacrylamide gel electrophoresis and western blotting. Explain the principles and perform fundamental protein fractionation, separation and purification techniques such as salt fractionation, size exclusion chromatography and ion exchange chromatography. Describe the principles underlying immunization strategies particularly as they relate to the production of monoclonal antibodies. Describe the principles involved in developing screening assays for monoclonal antibody production; then, coat plates with candidate antigens and perform the assays. 8

Credits:

2.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

kapiolani.hawaii.edu/page/home

Phone Number:

(808) 734-9000

Regional Accreditation:

Western Association of Schools and Colleges

Calendar System:

Semester

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