MLT 112 - Clinical Biochemistry I Spring

Institution:

Kapiolani Community College

Subject:

Description:

15 hours lecture/lab per week for 6 weeks Prerequisite(s): Credit or concurrent enrollment in CHEM 162/162L; admission to the MLT program or consent of MLT Program Director. Comment: MLT 112 is a modular course offered the last 6 weeks in the Spring semester only. Students will be expected to purchase latex or vinyl gloves for this course. MLT 112 introduces principles of clinical biochemistry pertaining to testing for chemical constituents in blood and body fluids. It covers general biochemistry of metabolism, carbohydrates, protein and enzymes. Student will practice techniques for spectrophotometry, glucose, protein, and protein fractionation and enzyme analysis. Upon successful completion of MLT 112, the student should be able to: Integrate knowledge of the theoretical principles of clinical biochemistry in laboratory diagnosis. Describe the metabolic pathways basic to the physiology of the human body. Describe the collection and handling of all clinical specimens to be processed for clinical chemistry. Describe the function, structure, mode of 2 action, and clinical significance of glucose, protein and protein fractions. Describe the theory underlying laboratory procedures for glucose, glycosylated glucose, protein, albumin, and protein fractionation by electrophoresis and chromatography. Correlate abnormalities of blood and urine chemistry associated with glucose and protein determinations. Explain enzyme kinetics and relate the concept to laboratory testing for enzymes. Calculate and prepare percent, normal and molar solutions and dilutions of concentrated solutions. Calculate mean and standard deviation and apply basic statistics to quality control in the chemistry laboratory. Use the appropriate statistical formula for determining reliability of clinical chemistry assays Perform the following manual clinical chemistry determinations on serum, plasma or urine within +/- two standard deviations of the stated value of the sample: a. Glucose b. Total Protein c. Albumin d. Protein Electrophoresis e. Other protein fractionation Operate and maintain according to standardized procedures and describe the principle of spectrophotometry. Utilize and calibrate serological and volumetric pipettors and micropipettors. Prepare written laboratory reports on each procedure performed and each instrument used. Perform all tests utilizing appropriate safety measures as stated in safety manuals. Organize their work in an orderly manner and maintain the laboratory area in a clean, working condition.

Credits:

3.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

kapiolani.hawaii.edu/page/home

Phone Number:

(808) 734-9000

Regional Accreditation:

Western Association of Schools and Colleges

Calendar System:

Semester

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