BIOL 275L - Cell and Molecular Biology Lab KCC AA/DY

Institution:

Kapiolani Community College

Subject:

Description:

4 hours lecture/lab per week Prerequisite(s): BIOL 171; BIOL 171L; BIOL 172; BIOL 172L; CHEM 272; credit or concurrent enrollment in BIOL 275; or instructor consent. Comment: BIOL 275L is cross-listed as MICR 240. BIOL 275L was formerly BIOL 270L. BIOL 275L is a lecture/laboratory in cell and molecular biology for life science majors. This course is taken either concurrently or after BIOL 275. Through lectures and laboratory exercises, students will acquire a fundamental understanding of the biochemistry of the cell. Students will also acquire competence in tissue culture and experience with modern advances in biotechnology and recombinant DNA technology. Upon successful completion of BIOL 275L the student should be able to: Prepare media and buffers needed for the culture of animal, plant and microalgae cells. Demonstrate proficiency in the specialized sterilization and quality control procedures used in a tissue culture laboratory. Demonstrate proficiency in routine cell culture protocols such as feeding schedules and medium supplements, subcultivation procedures, cell enumeration and viability testing, cryopreservation, and the detection and disposition of contaminated cultures. Demonstrate knowledge of the basic principles of protein chemistry by applying these principles in the designing and reporting of experiments utilizing enzymatic reactions, electrophoresis and immunoassays. Demonstrate knowledge of the basic principles of DNA structure, function, and chemistry by applying these principles in the designing and reporting of experiments utilizing DNA extraction and purification, electrophoresis, restriction enzyme analysis, DNA amplification, sequencing, and sequence analysis using standard bioinformatics databases and analysis protocols. Describe in detail the organization of life at the cellular and subcellular levels. Describe the structure and function of biological membranes and demonstrate an understanding of the processes which occur at the cell surface. Describe in detailed and specific terms the fundamental catabolic and anabolic metabolic processes that occur at the cellular level. Describe and experimentally manipulate the cytoskeleton particularly as it relates to intracellular traffic, cytokinesis and cell motility. Describe and experimentally manipulate the basic processes involved in cell signaling and the cell cycle and define the role of these processes in cell differentiation and in cancer. Describe the theories explaining the development of eukaryotes and the evolution of multicellular organisms. Describe and debate the ethical issues surrounding existing and proposed research and applications using living cells.

Credits:

2.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

kapiolani.hawaii.edu/page/home

Phone Number:

(808) 734-9000

Regional Accreditation:

Western Association of Schools and Colleges

Calendar System:

Semester

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