BIOL 3380 - Biochemistry Laboratory

Institution:

The University of Texas at Dallas

Subject:

Description:

Current techniques in the purification and characterization of enzymes to demonstrate fundamental principles that are utilized in modern biochemistry and molecular biology research laboratories. Practical skills taught include micropipetting, basic solution preparation, conducting pH measurements, isolating crude enzyme extracts, and performing standard activity assays. Advanced experiments with Green Fluorescent Protein and Lactate Dehydrogenase include Ni++-NTA affinity chromatography, ion chromatography, protein detection using Bradford, Lowry, and spectrophotometric assays, SDS-PAGE separation, Western Blot analysis, and enzyme kinetics. Prerequisite: BIOL 2281. Preor co-requisite: BIOL/CHEM 3361. (1-2) S

Credits:

3.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

www.utdallas.edu

Phone Number:

(972) 883-2111

Regional Accreditation:

Southern Association of Colleges and Schools

Calendar System:

Semester

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