BIOT 227 - Genetic Engineering and DNA Analysis

Institution:

Ivy Tech Community College-Central Office

Subject:

Biotechnology

Description:

Students will study the essential concepts and techniques in genetic engineering. Students will practice essential gene cloning procedures: isolation of DNA, restriction endonuclease digestion, agarose gel electrophoresis analysis, DNA ligation, and transformation into a host strain. Other essential techniques such as PCR, construction and screening of genomic or cDNA libraries, Southern and Northern blot analyses will be practiced. Students will understand the principles and ethical issues of animal or human cloning practices. Current methods for transfer and propagation of genes into plants and animals will be discussed. Various gene knockout techniques such as homologous gene recombination, site-directed mutagenesis, and RNAi will be introduced. The topics in genomics, proteomics, and bioinformatics will be discussed.

Credits:

4.00

Credit Hours:

Prerequisites:

Corequisites:

Exclusions:

Level:

Instructional Type:

Lecture

Notes:

Additional Information:

Historical Version(s):

Institution Website:

Phone Number:

Regional Accreditation:

North Central Association of Colleges and Schools

Calendar System:

Unaccounted for code

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